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Outer membrane vesicles facilitate trafficking of the hydrophobic signaling molecule CAI-1 between Vibrio harveyi cells

Brameyer, S; Plener, L; Müller, A; Klingl, A; Wanner, G; Jung, K; (2018) Outer membrane vesicles facilitate trafficking of the hydrophobic signaling molecule CAI-1 between Vibrio harveyi cells. Journal of Bacteriology , 200 (15) , Article e00740-17. 10.1128/JB.00740-17. Green open access

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Abstract

Many bacteria use extracellular signaling molecules to coordinate group behavior, a process referred to as quorum sensing (QS). However, some QS molecules are hydrophobic in character and are probably unable to diffuse across the bacterial cell envelope. How these molecules are disseminated between bacterial cells within a population is not yet fully understood. Here we show that the marine pathogenVibrio harveyipackages the hydrophobic QS molecule CAI-1, a long-chain amino ketone, into outer membrane vesicles. Electron micrographs indicate that outer membrane vesicles of variable size are predominantly produced and released into the surroundings during stationary phase ofV. harveyi, which correlates with the timing of CAI-1-dependent signaling. The large vesicles (diameter < 55 nm) can trigger a QS phenotype in CAI-1 non-producingV. harveyiandV. choleraecells. Packaging of CAI-1 into outer membrane vesicles might stabilize the molecule in aqueous environments and facilitate its distribution over distances.IMPORTANCEFormation of membrane vesicles is ubiquitous among bacteria. These vesicles are involved in protein and DNA transfer and offer new approaches for vaccination. Gram-negative bacteria use among others hydrophobic signaling molecules for cell-cell communication, however due to their hydrophobic character it is unclear how these molecules are disseminated between bacterial cells. Here we show that the marineVibrio harveyipackages one of its quorum sensing molecules, the long-chain ketone CAI-1, into outer membrane vesicles (OMVs). Isolated CAI-1-containing vesicles trigger a quorum sensing phenotype in CAI-1 non-producingV. harveyiand also inV. choleraecells. Packaging of CAI-1 into OMVs not only solubilize, stabilize and concentrate this class of molecules, but facilitate their distribution between bacteria that live in aqueous environments.

Type: Article
Title: Outer membrane vesicles facilitate trafficking of the hydrophobic signaling molecule CAI-1 between Vibrio harveyi cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1128/JB.00740-17
Publisher version: http://doi.org/10.1128/JB.00740-17
Language: English
Additional information: Copyright © 2018 American Society for Microbiology. This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10045901
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