Culley, S;
Albrecht, D;
Jacobs, C;
Pereira, PM;
Leterrier, C;
Mercer, J;
Henriques, R;
(2018)
Quantitative mapping and minimization of super-resolution optical imaging artifacts.
Nature Methods
, 15
pp. 263-266.
10.1038/nmeth.4605.
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Abstract
Super-resolution microscopy depends on steps that can contribute to the formation of image artifacts, leading to misinterpretation of biological information. We present NanoJ-SQUIRREL, an ImageJ-based analytical approach that provides quantitative assessment of super-resolution image quality. By comparing diffraction-limited images and super-resolution equivalents of the same acquisition volume, this approach generates a quantitative map of super-resolution defects and can guide researchers in optimizing imaging parameters.
| Type: | Article |
|---|---|
| Title: | Quantitative mapping and minimization of super-resolution optical imaging artifacts |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/nmeth.4605 |
| Publisher version: | http://dx.doi.org/10.1038/nmeth.4605 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10044159 |
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