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Nano-engineered microcapsules boost the treatment of persistent pain

Kopach, O; Zheng, K; Dong, L; Sapelkin, A; Voitenko, N; Sukhorukov, GB; Rusakov, DA; (2018) Nano-engineered microcapsules boost the treatment of persistent pain. Drug Delivery , 25 (1) pp. 435-447. 10.1080/10717544.2018.1431981. Green open access

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Abstract

Persistent pain remains a major health issue: common treatments relying on either repeated local injections or systemic drug administration are prone to concomitant side-effects. It is thought that an alternative could be a multifunctional cargo system to deliver medicine to the target site and release it over a prolonged time window. We nano-engineered microcapsules equipped with adjustable cargo release properties and encapsulated the sodium-channel blocker QX-314 using the layer-by-layer (LbL) technology. First, we employed single-cell electrophysiology to establish in vitro that microcapsule application can dampen neuronal excitability in a controlled fashion. Secondly, we used two-photon excitation imaging to monitor and adjust long-lasting release of encapsulated cargo in target tissue in situ. Finally, we explored an established peripheral inflammation model in rodents to find that a single local injection of QX-314-containing microcapsules could provide robust pain relief lasting for over a week. This was accompanied by a recovery of the locomotive deficit and the amelioration of anxiety in animals with persistent inflammation. Post hoc immunohistology confirmed biodegradation of microcapsules over a period of several weeks. The overall remedial effect lasted 10–20 times longer than that of a single focal drug injection. It depended on the QX-314 encapsulation levels, involved TRPV1-channel-dependent cell permeability of QX-314, and showed no detectable side-effects. Our data suggest that nano-engineered encapsulation provides local drug delivery suitable for prolonged pain relief, which could be highly advantageous compared to existing treatments.

Type: Article
Title: Nano-engineered microcapsules boost the treatment of persistent pain
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/10717544.2018.1431981
Publisher version: https://doi.org/10.1080/10717544.2018.1431981
Language: English
Additional information: Copyright © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Biodegradable microcapsules, persistent pain, Na+ channels, drug diffusion, neuronal excitability, pain relief, locomotive deficit and anxiety
URI: http://discovery.ucl.ac.uk/id/eprint/10043302
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