Schwarz, D;
Kollo, M;
Bosch, C;
Feinauer, C;
Whiteley, I;
Margrie, TW;
Cutforth, T;
(2018)
Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.
Nature Communications
, 9
, Article 183. 10.1038/s41467-017-02560-7.
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Abstract
Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the “inputs” and “outputs,” remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory glomerulus. This allows us to detect sparse higher-order features of the wiring architecture that are inaccessible to statistical labeling approaches. Thus, NEM labeling provides crucial complementary information to dense circuit reconstruction techniques. Relying solely on targeting an electrode to the region of interest and passive biophysical properties largely common across cell types, this can easily be employed anywhere in the CNS.
| Type: | Article |
|---|---|
| Title: | Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41467-017-02560-7 |
| Publisher version: | https://doi.org/10.1038/s41467-017-02560-7 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, MOUSE OLFACTORY-BULB, SINGLE-CELL ELECTROPORATION, EXTERNAL PLEXIFORM LAYER, LOCAL NEURONAL CIRCUITS, TUFTED CELLS, GRANULE CELLS, IN-VIVO, SYNAPTIC CIRCUITS, CORTICAL-NEURONS, RECONSTRUCTION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10042308 |
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