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A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry

Welker, KM; Lassetter, B; Brandes, CM; Prasad, S; Koop, DR; Mehta, PH; (2016) A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry. Psychoneuroendocrinology , 71 pp. 180-188. 10.1016/j.psyneuen.2016.05.022. Green open access

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Abstract

Enzyme immunoassays (EIAs) are widely used to measure salivary testosterone. However, little is known about how accurately different EIAs assess testosterone, partially because estimates across various EIAs differ considerably. We compared testosterone concentrations across EIAs of three commonly used manufacturers (DRG International, Salimetrics, and IBL International) to liquid chromatography tandem mass spectrometry (LC–MS/MS). Relative to EIAs from Salimetrics and IBL International, EIAs supplied by DRG International provided the closest approximation to LC–MS/MS testosterone concentrations, followed closely by EIAs from Salimetrics, and then IBL. Additionally, EIAs tended to inflate estimates of lower testosterone concentrations in women. Examining our results and comparing them to existing data revealed that testosterone EIAs had decreased linear correspondence with LC–MS/MS in comparison to cortisol EIAs. Overall, this paper provides researchers with information to better measure testosterone in their research and more accurately compare testosterone measurements across different methods.

Type: Article
Title: A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.psyneuen.2016.05.022
Publisher version: https://doi.org/10.1016/j.psyneuen.2016.05.022
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism, Neurosciences, Psychiatry, Neurosciences & Neurology, Salivary testosterone, Immunoassays, Liquid chromatography, Mass spectrometry, Measurement, AGGRESSIVE-BEHAVIOR, CORTISOL, DOMINANCE, MEN
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Div of Psychology and Lang Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Div of Psychology and Lang Sciences > Experimental Psychology
URI: http://discovery.ucl.ac.uk/id/eprint/10040152
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