O'Farrell, FM;
Mastitskaya, S;
Hammond-Haley, M;
Freitas, F;
Wah, WR;
Attwell, D;
(2017)
Capillary pericytes mediate coronary no-reflow after myocardial ischaemia.
elife
, 6
, Article e29280. 10.7554/eLife.29280.
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Abstract
After cardiac ischaemia, a prolonged decrease of coronary microvascular perfusion often occurs even after flow is restored in an upstream artery. This 'no-reflow' phenomenon worsens patient prognosis. In the brain, after stroke, a similar post-ischaemic 'no-reflow' has been attributed to capillary constriction by contractile pericytes. We now show that occlusion of a rat coronary artery, followed by reperfusion, blocks 40% of cardiac capillaries and halves perfused blood volume within the affected region. Capillary blockages colocalised strongly with pericytes, where capillary diameter was reduced by 37%. The pericyte relaxant adenosine increased capillary diameter by 21% at pericyte somata, decreased capillary block by 25% and increased perfusion volume by 57%. Thus, cardiac pericytes constrict coronary capillaries and reduce microvascular blood flow after ischaemia, despite re-opening of the culprit artery. Cardiac pericytes are therefore a novel therapeutic target in ischaemic heart disease.
| Type: | Article |
|---|---|
| Title: | Capillary pericytes mediate coronary no-reflow after myocardial ischaemia. |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7554/eLife.29280 |
| Publisher version: | http://dx.doi.org/10.7554/eLife.29280 |
| Language: | English |
| Additional information: | © 2017 eLife Sciences Publications Ltd. Subject to a Creative Commons Attribution license, except where otherwise noted. This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Cell biology, human biology, medicine, rat |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10038809 |
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