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Gas chromatographic-mass spectrometric assay to measure urinary N tau-methylhistamine excretion in man.

Murray, S; Wellings, R; Taylor, IK; Fuller, RW; Taylor, GW; (1991) Gas chromatographic-mass spectrometric assay to measure urinary N tau-methylhistamine excretion in man. J Chromatogr , 567 (2) pp. 289-298.

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Abstract

An assay has been developed for N tau-methylhistamine, a major metabolite of the autocoid histamine, based on gas chromatography-electron-capture negative-ion chemical ionisation mass spectrometry. N tau-Methylhistamine was extracted from urine by cation-exchange chromatography and converted to its di-(3,5-bistrifluoromethylbenzoyl) derivative. The latter has good chromatographic properties and gives a negative-ion mass spectrum with the molecular ion (M., m/z 605) as base peak. A commercially available trideuterated analogue of N tau-methylhistamine was used as internal standard. Basal urinary excretion of N tau-methylhistamine in five normal subjects was found to be 0.21 +/- 0.05 mumol/h (289 +/- 74 mumol/mol of creatinine). This value was not significantly altered in these subjects following the infusion of a subpharmacological dose of histamine. In eight atopic volunteers, basal urinary excretion of N tau-methylhistamine was also not significantly changed following challenge with inhaled allergen.

Type: Article
Title: Gas chromatographic-mass spectrometric assay to measure urinary N tau-methylhistamine excretion in man.
Location: Netherlands
Keywords: Adult, Allergens, Chromatography, Ion Exchange, Female, Gas Chromatography-Mass Spectrometry, Histamine, Humans, Indicators and Reagents, Male, Methylhistamines, Reference Standards
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/10036305
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