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The inositol pyrophosphate synthesis pathway in Trypanosoma brucei is linked to polyphosphate synthesis in acidocalcisomes

Cordeiro, CD; Saiardi, A; Docampo, R; (2017) The inositol pyrophosphate synthesis pathway in Trypanosoma brucei is linked to polyphosphate synthesis in acidocalcisomes. Molecular Microbiology , 106 (2) pp. 319-333. 10.1111/mmi.13766. Green open access

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Abstract

Inositol pyrophosphates are novel signaling molecules possessing high-energy pyrophosphate bonds and involved in a number of biological functions. Here, we report the correct identification and characterization of the kinases involved in the inositol pyrophosphate biosynthetic pathway in Trypanosoma brucei: inositol polyphosphate multikinase (TbIPMK), inositol pentakisphosphate 2-kinase (TbIP5K) and inositol hexakisphosphate kinase (TbIP6K). TbIP5K and TbIP6K were not identifiable by sequence alone and their activities were validated by enzymatic assays with the recombinant proteins or by their complementation of yeast mutants. We also analyzed T. brucei extracts for the presence of inositol phosphates using polyacrylamide gel electrophoresis and high-performance liquid chromatography. Interestingly, we could detect inositol phosphate (IP), inositol 4,5-bisphosphate (IP2), inositol 1,4,5-trisphosphate (IP3), and inositol hexakisphosphate (IP6) in T. brucei different stages. Bloodstream forms unable to produce inositol pyrophosphates, due to downregulation of TbIPMK expression by conditional knockout, have reduced levels of polyphosphate and altered acidocalcisomes. Our study links the inositol pyrophosphate pathway to the synthesis of polyphosphate in acidocalcisomes, and may lead to better understanding of these organisms and provide new targets for drug discovery.

Type: Article
Title: The inositol pyrophosphate synthesis pathway in Trypanosoma brucei is linked to polyphosphate synthesis in acidocalcisomes
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/mmi.13766
Publisher version: http://dx.doi.org/10.1111/mmi.13766
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: HEXAKISPHOSPHATE KINASE 1, EUKARYOTIC PHOSPHATE HOMEOSTASIS, BLOOD-STREAM FORMS, DIPHOSPHOINOSITOL POLYPHOSPHATES, HYPEROSMOTIC STRESS, PHOSPHOLIPASE-C, TELOMERE LENGTH, HUMAN HOMOLOG, CELL-DEATH, IN-VIVO, Acidocalcisome, polyphosphate, inositol pyrophosphate, calcium, Trypanosoma brucei
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/10030561
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