UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Nucleation and growth of microbial lipase crystals from clarified concentrated fermentation broths

Jacobsen, C; Garside, J; Hoare, M; (1998) Nucleation and growth of microbial lipase crystals from clarified concentrated fermentation broths. Biotechnology and Bioengineering , 57 (6) pp. 666-675. 10.1002/(SICI)1097-0290(19980320)57:6<666::AID-BIT4>3.0.CO;2-J.

Full text not available from this repository.

Abstract

Bulk crystallization is emerging as a new industrial operation for protein recovery. Characterization of bulk protein crystallization is more complex than protein crystallization for structural study where single crystals are grown in flow cells. This is because both nucleation and crystal growth processes are taking place while the supersaturation falls. An algorithm is presented to characterize crystallization using the rates of the two kinetic processes, nucleation and growth. The values of these rates allow ready comparison of the crystallization process under different operating conditions. The crystallization, via adjustment to the isoelectric pH of a fungal lipase from clarified fermentation broth, is described for a batch stirred reactor. A maximum nucleation rate of five to six crystals formed per microliter of suspension per second and a high power dependency (≈11) on the degree of supersaturation were found. The suspended protein crystals were found to grow at a rate of up to 15-20 nm/s and also to exhibit a high power dependency (≈6) of growth rate on the degree of supersaturation.

Type: Article
Title: Nucleation and growth of microbial lipase crystals from clarified concentrated fermentation broths
DOI: 10.1002/(SICI)1097-0290(19980320)57:6<666::AID-BIT4>3.0.CO;2-J
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/10029039
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item